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Article

Transposon Tn917PF1 mutagenesis in Bacillus licheniformis.

Microbiology (Reading, England) - 1 Nov 1994

Prágái Z, Trân S L, Nagy T, Fülöp L, Holczinger A, Sik T

Abstract excerpt

The plasmid pTnPFl containing the transposon Tn917PF1 was introduced into the protoplasts of two Bacillus licheniformis strains in the presence of polyethylene glycol. Transpositions were produced at high temperature which inhibited plasmid replication and kanamycin was used for selection.Transposon Tn917PF1 was inserted randomly into the bacterial chromosome,producing different auxotrophic, prophage BLF and...

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PMID: 19810283
DOI: 10.1099/13500872-140-11-3091

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