Article
Characterization of transformation-deficient mutants of Acinetobacter calcoaceticus.
Molecular microbiology - 1 Jul 1992
Palmen R, Vosman B, Kok R, van der Zee J R, Hellingwerf K J
Abstract excerpt
Three Acinetobacter calcoaceticus transformation-deficient mutants, obtained by insertional mutagenesis with the nptll gene, have been characterized physiologically. One mutant (AAC211) was found to be completely transformation deficient, while two others, AAC213 and AAC214, were severely impaired in transformation efficiency (100-1000 times lower than the wild type). The latter applied to both chromosomal as...
Topics
- Acinetobacter calcoaceticus
- Chromosomes, Bacterial
- Cloning, Molecular
- Genes, Bacterial
- Mutagenesis, Insertional
- Mutation
- Plasmids
- Rec A Recombinases
- Transformation, Bacterial