Article
Direct evidence that restriction endonucleases may under estimate the degree of divergence between molecules.
Current genetics - 1 Aug 1990
De Giorgi C, De Luca F, Stefanile C, Pesole G, Saccone C
Abstract excerpt
We studied two polymorphic forms of mtDNA extracted from A. lixula eggs. In order to compare and to quantitate the variability, we sequenced specific regions of the two molecules. In this way, we obtained a precise measurement of the variability within two haplotypes. We also obtained a direct demonstration that some differences in nucleotide sequence can escape detection when restriction endonuclease analysis is...
Topics
- Animals
- Base Sequence
- DNA Restriction Enzymes
- DNA, Mitochondrial
- Genetic Variation
- Haplotypes
- Polymorphism, Restriction Fragment Length
- Restriction Mapping
- Sea Urchins