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Article

Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant.

Bioresource technology - 1 Jan 2010

Ma Yuchao, Yu Huimin, Pan Wenyu, Liu Changchun, Zhang Shuliang, Shen Zhongyao

Abstract excerpt

Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative...

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PMID: 19720524
DOI: 10.1016/j.biortech.2009.07.057

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