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Article

A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis.

Diagnostic microbiology and infectious disease - 1 Sept 2009

Ciammaruconi Andrea, Grassi Saverio, Faggioni Giovanni, De Santis Riccardo, Pittiglio Valentina, D'Amelio Raffaele, Vergnaud Gilles, Lista Florigio

Abstract excerpt

Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing...

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Identifiers

PMID: 19679229
DOI: 10.1016/j.diagmicrobio.2009.04.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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