Article
Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology.
The Journal of infectious diseases - 1 Feb 1990
Arbeit R D, Arthur M, Dunn R, Kim C, Selander R K, Goldstein R
Abstract excerpt
Escherichia coli infecting the urinary tract and other extraintestinal sites are often identical in many phenotypic and genotypic characteristics. To discriminate among pathogenic E. coli isolates, chromosomal DNA was digested with restriction endonucleases that recognize eight base pair sequences and the resulting 20-25 fragments were resolved by pulsed field gel electrophoresis (PFGE). Different restriction...
Topics
- Biliary Tract Diseases
- Blotting, Southern
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Escherichia coli
- Escherichia coli Infections
- Genotype
- Humans
- Phenotype
- Polymorphism, Restriction Fragment Length
