Article
Rapid determination of Escherichia coli O157:H7 lineage types and molecular subtypes by using comparative genomic fingerprinting.
Applied and environmental microbiology - 1 Nov 2008
Laing Chad, Pegg Crystal, Yawney Davis, Ziebell Kim, Steele Marina, Johnson Roger, Thomas James E, Taboada Eduardo N, Zhang Yongxiang, Gannon Victor P J
Abstract excerpt
In this study, variably absent or present (VAP) regions discovered through comparative genomics experiments were targeted for the development of a rapid, PCR-based method to subtype and fingerprint Escherichia coli O157:H7. Forty-four VAP loci were analyzed for discriminatory power among 79 E. coli O157:H7 strains of 13 phage types (PT). Twenty-three loci were found to maximize resolution among strains,...
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