Article
Analysis of protein expression in response to osmotic stress in Escherichia coli.
FEMS microbiology letters - 1 Nov 1990
Bostford J L
Abstract excerpt
Two strains of Escherichia coli isogenic except for the cya (adenylate cyclase) allele were grown with [35S]methionine and cysteine in minimal defined glucose medium and in this medium with 600 mM NaCl to induce osmotic stress. Cells were grown for approximately two generations. The labeled proteins were separated by 2-dimensional electrophoresis and were quantified fluorographically. Of the 263 major proteins...
Topics
- Adenylyl Cyclases
- Alleles
- Bacterial Proteins
- Cyclic AMP
- Escherichia coli
- Methionine
- Osmotic Pressure
- Sodium Chloride