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Article

A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli.

Metabolic engineering - 1 Jul 2000

Gill R T, Valdes J J, Bentley W E

Abstract excerpt

Global gene regulation throughout the Escherichia coli stress response to overexpression of each of five recombinant proteins was evaluated. Reverse-transcriptase polymerase chain reaction-amplified mRNA from induced and control cells were hybridized with a DNA array of Kohara clones representing 16% (700 genes) of the E. coli genome. Subsequently, Northern analysis was performed for quantification of specific...

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Identifiers

PMID: 11056060
DOI: 10.1006/mben.2000.0148

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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