Article
The absence of an identifiable single catalytic base residue in Thermobifida fusca exocellulase Cel6B.
The FEBS journal - 1 Jul 2009
Vuong Thu V, Wilson David B
Abstract excerpt
Thermobifida fusca exocellulase Cel6B acts by an inverting hydrolysis mechanism; however, the catalytic acid and base residues for this enzyme have not been confirmed. Site-directed mutagenesis and kinetic studies were used to show that Asp274 is the catalytic acid, which is consistent with what is found for other members of family-6 glycoside hydrolases; however, a single catalytic base was not identified....
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