Article
Application of a monoclonal antibody specific for the delta chain of hemoglobin A2 in the diagnosis of beta thalassemia.
American journal of hematology - 1 Nov 1991
Shyamala M, Kiefer C R, Moscoso H, Garver F A
Abstract excerpt
We have developed a murine monoclonal antibody (mAb) specific for the delta chain of hemoglobin (Hb) A2 that does not cross-react with alpha, beta, or gamma chains. The mAb reacted with Hb P-Nilotic (beta delta hybrid), but not with Hb Lepore-Boston (delta beta hybrid), indicating an epitope consisting of positions 116 (Arg) and 117 (Asn) or 125 (Gln) and 126 (Met) of the delta chain. By using this antibody, we...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Enzyme-Linked Immunosorbent Assay
- Hemoglobin A2
- Hemolysis
- Humans
- Mice
- Mice, Inbred BALB C
- Phenotype
- Thalassemia