Article
RNA interference-mediated silencing of the respiratory syncytial virus nucleocapsid defines a potent antiviral strategy.
Antimicrobial agents and chemotherapy - 1 Sept 2009
Alvarez Rene, Elbashir Sayda, Borland Todd, Toudjarska Ivanka, Hadwiger Philipp, John Mathias, Roehl Ingo, Morskaya Svetlana Shulga, Martinello Rick, Kahn Jeffrey, Van Ranst Mark, Tripp Ralph A, DeVincenzo John P, Pandey Rajendra, Maier Martin, Nechev Lubomir, Manoharan Muthiah, Kotelianski Victor, Meyers Rachel
Abstract excerpt
We describe the design and characterization of a potent human respiratory syncytial virus (RSV) nucleocapsid gene-specific small interfering RNA (siRNA), ALN-RSV01. In in vitro RSV plaque assays, ALN-RSV01 showed a 50% inhibitory concentration of 0.7 nM. Sequence analysis of primary isolates of RSV showed that the siRNA target site was absolutely conserved in 89/95 isolates, and ALN-RSV01 demonstrated activity...
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