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Multiplex, Quantitative, Real-Time PCR Assay for Cytomegalovirus and Human DNA

1 Jul 2002

Abstract excerpt

We created a multiplex, quantitative, real-time PCR assay that amplifies cytomegalovirus (CMV) and human DNA in the same reaction tube, allowing for a viral load determination that is normalized to measured human DNA. The assay targets a conserved region of the CMV DNA polymerase gene that is not affected by known drug resistance mutations. All 36 strains of CMV detected by culture or qualitative PCR in a...

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PMID: 12089251
DOI: 10.1128/jcm.40.7.2381-2386.2002

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