Article
[A variant of DDRT-PCR using anchored mini-exon primers for identification of differentially expressed sequences in Leishmania infantum].
Archives de l'Institut Pasteur de Tunis - 1 Jan 2008
Turki-Mannoubi L, Kbaier-Hachemi H, Barhoumi M, Chakroun A S, Guizani I
Abstract excerpt
Leishmania infantum (L.i) is responsible for visceral (VL) or cutaneous (CL) leishmaniasis. Previous studies done in Honduras by differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) failed to demonstrate differences in expression profiles among L. infantum VL and CL parasites. For purpose of comparing expression among L. infantum isolates in Tunisia, a variant of this technique adapted...
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