Article
Quantitative analysis of synaptic boutons in Drosophila primary neuronal cultures.
Brain research - 14 Jul 2009
Darya Kauroon, Ganguly Archan, Lee Daewoo
Abstract excerpt
Little information is currently available for structural and quantitative aspects of Drosophila central synapses due to difficulties in accessing those synapses in the tiny fly brain. Here, we developed a new approach to quantitatively analyze central synapses using Drosophila primary neuronal cultures. Two different markers were used to identify synaptic boutons: GFP marking with a synaptotagmin (Syt)::eGFP...
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