Article
Use of the asymmetric polymerase chain reaction and DNA sequencing to determine genetic variability of bean golden mosaic geminivirus in the Dominican Republic.
The Journal of general virology - 1 Nov 1991
Gilbertson R L, Rojas M R, Russell D R, Maxwell D P
Abstract excerpt
A combination of the polymerase chain reaction (PCR), asymmetric PCR (A-PCR) and DNA sequencing was used to determine the nucleotide sequence of a hypervariable region of the bipartite genome of bean golden mosaic geminivirus (BGMV). This region, which was part of the intergenic region of the DNA...
Topics
- Base Sequence
- DNA, Viral
- Dominican Republic
- Fabaceae
- Genetic Variation
- Molecular Sequence Data
- Mosaic Viruses
- Plants, Medicinal
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Nucleic Acid