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Article

Identification and prevention of genotyping errors caused by G-quadruplex- and i-motif-like sequences.

Clinical chemistry - 1 Jul 2009

Wenzel Jürgen J, Rossmann Heidi, Fottner Christian, Neuwirth Stefan, Neukirch Carolin, Lohse Peter, Bickmann Julia K, Minnemann Timo, Musholt Thomas J, Schneider-Rätzke Brigitte, Weber Matthias M, Lackner Karl J

Abstract excerpt

BACKGROUND: Reliable PCR amplification of DNA fragments is the prerequisite for most genetic assays. We investigated the impact of G-quadruplex- or i-motif-like sequences on the reliability of PCR-based genetic analyses. METHODS: We found the sequence context of a common intronic polymorphism in the MEN1 gene (multiple endocrine neoplasia I) to be the cause of systematic genotyping errors by inducing preferential...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19406917
DOI: 10.1373/clinchem.2008.118661

Genes

ADO
MEN1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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