Article
Rapid decline in the efficiency of HIV drug resistance genotyping from dried blood spots (DBS) and dried plasma spots (DPS) stored at 37 degrees C and high humidity.
The Journal of antimicrobial chemotherapy - 1 Jul 2009
García-Lerma J Gerardo, McNulty Amanda, Jennings Cheryl, Huang Diana, Heneine Walid, Bremer James W
Abstract excerpt
OBJECTIVES: Dried blood spots (DBS) and dried plasma spots (DPS) are considered convenient alternatives to serum and plasma for HIV drug resistance testing in resource-limited settings. We sought to investigate how extreme conditions could affect the short-term ability to amplify and genotype HIV from DBS. METHODS: A panel of six matched DPS/DBS was generated using blood collected from HIV-infected donors....
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