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Mesenchymal stem cells facilitate the derivation of human embryonic stem cells from cryopreserved poor-quality embryos

28 Apr 2009

Abstract excerpt

BACKGROUND: Human embryonic stem cells (hESCs) have opened up a new area of research in biomedicine. The efficiency of hESC derivation from frozen poor-quality embryos is low and normally achieved by plating embryos on mouse or human foreskin feeders (HFFs). We attempted to optimize embryo survival and hESC derivation. METHODS: Three conditions were tested on frozen poor-quality embryos: (i) embryo treatment with...

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PMID: 19401322
DOI: 10.1093/humrep/dep107

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