Article
A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
30 Jun 2003
Abstract excerpt
BACKGROUND: Human embryonic stem (hES) cell lines were first cultured using fetal mouse fibroblasts as feeder cells. To avoid feeders and to reduce the amount of xeno-components, Matrigel- and laminin-coated dishes, and conditioned mouse feeder cell medium have been used, and hES cells have also been cultured on human fetal muscle and skin, and adult Fallopian tube epithelial cells. METHODS: We used post-natal,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.