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Derivation of a diploid human embryonic stem cell line from a mononuclear zygote

29 Jan 2004

Abstract excerpt

BACKGROUND: IVF occasionally produces aneuploid zygotes with one or three pronuclei (PN). Routinely, these zygotes are discarded. The aim of this work was to establish human embryonic stem cell (hESC) lines from blastocysts resulting from abnormal fertilization. METHODS: Abnormally fertilized zygotes were cultured to the blastocyst stage and, following zona pellucida digestion, zona-free blastocysts were placed...

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PMID: 14998969
DOI: 10.1093/humrep/deh135

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