Real-time detection of cofactor availability in genetically modified living Saccharomyces cerevisiae cells--simultaneous probing of different geno- and phenotypes.

Abstract excerpt

This work describes a mediated amperometric method for simultaneous real-time probing of the NAD(P)H availability in two different phenotypes, fermentative and respiratory, of the phosphoglucose isomerase deletion mutant strain of S. cerevisiae, EBY44 [ENY.WA-1A pgi1-1D::URA3], and its parental strain, ENY.WA-1A. The developed method is based on multichannel detection using microelectrode arrays. Its versatility...

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