Article
Genomic DNA enrichment using sequence capture microarrays: a novel approach to discover sequence nucleotide polymorphisms (SNP) in Brassica napus L.
PloS one - 1 Jan 2013
Clarke Wayne E, Parkin Isobel A, Gajardo Humberto A, Gerhardt Daniel J, Higgins Erin, Sidebottom Christine, Sharpe Andrew G, Snowdon Rod J, Federico Maria L, Iniguez-Luy Federico L
Abstract excerpt
Targeted genomic selection methodologies, or sequence capture, allow for DNA enrichment and large-scale resequencing and characterization of natural genetic variation in species with complex genomes, such as rapeseed canola (Brassica napus L., AACC, 2n=38). The main goal of this project was to combine sequence capture with next generation sequencing (NGS) to discover single nucleotide polymorphisms (SNPs) in...
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