Article
Cellular phenotypes of human model neurons (NT2) after differentiation in aggregate culture.
Cell and tissue research - 1 Jun 2009
Podrygajlo Grzegorz, Tegenge Million A, Gierse Andrea, Paquet-Durand Francois, Tan Saime, Bicker Gerd, Stern Michael
Abstract excerpt
The well-characterized human teratocarcinoma line Ntera2 (NT2) can be differentiated into mature neurons. We have significantly shortened the time-consuming process for generating postmitotic neurons to approximately 4 weeks by introducing a differentiation protocol for free-floating cell aggregates and a subsequent purification step. Here, we characterize the neurochemical phenotypes of the neurons derived from...
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