Article
Cellular phenotypes of human model neurons (NT2) after differentiation in aggregate culture.
Cell and tissue research - 1 Jun 2009
Podrygajlo Grzegorz, Tegenge Million A, Gierse Andrea, Paquet-Durand Francois, Tan Saime, Bicker Gerd, Stern Michael
Abstract excerpt
The well-characterized human teratocarcinoma line Ntera2 (NT2) can be differentiated into mature neurons. We have significantly shortened the time-consuming process for generating postmitotic neurons to approximately 4 weeks by introducing a differentiation protocol for free-floating cell aggregates and a subsequent purification step. Here, we characterize the neurochemical phenotypes of the neurons derived from...
Topics
- Animals
- Cell Aggregation
- Cell Culture Techniques
- Cell Differentiation
- Cell Proliferation
- Cyclic GMP
- Cytoskeleton
- Dopamine
- Fluorescent Antibody Technique
- Humans
- Mice
- Neurons
- Neurotransmitter Agents
- Nitric Oxide
- Nitric Oxide Donors
- Phenotype
- Tyrosine 3-Monooxygenase