Article
A novel nicotine catabolic plasmid pMH1 in Pseudomonas sp. strain HF-1.
Canadian journal of microbiology - 1 Mar 2009
Wang Meizhen, Yang Guiqin, Min Hang, Lv Zhenmei
Abstract excerpt
Attempts were made to acquire a plasmid-loss mutant via various methods (spontaneous mutation, SDS, and mitomycin C), among which the method involving mitomycin C (10 microg/mL) has been proven successful. Concomitant with the loss of the plasmid in Pseudomonas sp. strain HF-1, the cured derivative was identified as having a nicotine-negative (Nic-) phenotype, named mutant strain 6-13 (Nic-). After plasmids were...
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