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Challenging loop—mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii

1 May 2015

Abstract excerpt

OBJECTIVE: To compare analytical sensitivity and specificity of a newly described DNA amplification technique, LAMP and nested PCR assay targeting the RE and B1 genes for the detection of Toxoplasma gondii (T. gondii) DNA. METHODS: The analytical sensitivity of LAMP and nested-PCR was obtained against10-fold serial dilutions of T. gondii DNA ranging from 1 ng to 0.01 fg. DNA samples of other parasites and human...

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PMID: 26003595
DOI: 10.1016/s1995-7645(14)60345-x

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