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Triplex PCR using new primers for the detection of Toxoplasma gondii

25 Apr 2012

Abstract excerpt

Molecular methods are used increasingly for the detection of Toxoplasma gondii infection. This study developed a rapid, sensitive, and specific conventional triplex PCR for the detection of the B1 gene and ITS1 region of T. gondii using newly designed primers and an internal control based on the Vibrio cholerae HemM gene. The annealing temperature and concentrations of the primers, MgCl(2), and dNTPs were...

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PMID: 22561042
DOI: 10.1016/j.exppara.2012.04.009

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