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Article

Improvement of crystal quality by surface mutations of beta-lactamase Toho-1.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Apr 2009

Shimamura Tatsuro, Nitanai Yasushi, Uchiyama Takuro, Matsuzawa Hiroshi

Abstract excerpt

The beta-lactamase Toho-1 exhibits a strong tendency to form merohedrally twinned crystals. Here, the crystal quality of Toho-1 was improved by using surface modification to remove a sulfate ion involved in crystal packing. The surface-modified Toho-1 variant (R274N/R276N) was crystallized under similar conditions to those used for wild-type Toho-1. R274N/R276N did not form merohedrally twinned crystals. The...

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Identifiers

PMID: 19342785
DOI: 10.1107/S1744309109008240
PMCID: PMC2664765

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Source receipt: pubmed, retrieved 7 Sept 2026.

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