Article
Detection of unknown deletions in beta-globin gene cluster using relative quantitative PCR methods.
European journal of haematology - 1 Sept 2009
Babashah Sadegh, Jamali Somayeh, Mahdian Reza, Nosaeid Mina Hayat, Karimipoor Morteza, Alimohammadi Raheleh, Raeisi Marzieh, Maryami Fereshteh, Masoudifar Mahboubeh, Zeinali Sirous
Abstract excerpt
beta-Thalassemia is mainly caused by mutations involving single base substitution and small deletions. However, a considerable number of carriers are suspected to have large deletions in beta-globin gene cluster. Common strategy for identifying deletions with definite breakpoints is based on Gap PCR. There are, however, some cases with indefinite breakpoints which usually cannot be detected by this method. We...
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