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Development of Loop-Mediated Isothermal Amplification Assay for Detection of <i>Entamoeba histolytica</i>

25 Mar 2009

Abstract excerpt

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay....

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PMID: 19321720
DOI: 10.1128/jcm.00105-09

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