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Real-Time PCR for Detection and Differentiation of<i>Entamoeba histolytica</i>and<i>Entamoeba dispar</i>in Fecal Samples

26 Nov 2002

Abstract excerpt

A closed-tube, real-time PCR assay was developed for sensitive and specific detection and differentiation of the two closely related intestinal protozoan parasites Entamoeba histolytica and Entamoeba dispar directly from human feces. The assay is performed with the LightCycler system using fluorescence-labeled detection probes and primers amplifying a 310-bp fragment from the high-copy-number, ribosomal...

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PMID: 12454128
DOI: 10.1128/jcm.40.12.4413-4417.2002

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