Article
The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy.
The Journal of clinical investigation - 1 Apr 2009
Montini Eugenio, Cesana Daniela, Schmidt Manfred, Sanvito Francesca, Bartholomae Cynthia C, Ranzani Marco, Benedicenti Fabrizio, Sergi Lucia Sergi, Ambrosi Alessandro, Ponzoni Maurilio, Doglioni Claudio, Di Serio Clelia, von Kalle Christof, Naldini Luigi
Abstract excerpt
gamma-Retroviral vectors (gammaRVs), which are commonly used in gene therapy, can trigger oncogenesis by insertional mutagenesis. Here, we have dissected the contribution of vector design and viral integration site selection (ISS) to oncogenesis using an in vivo genotoxicity assay based on transplantation of vector-transduced tumor-prone mouse hematopoietic stem/progenitor cells. By swapping genetic elements...
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