Article
Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype.
Journal of clinical microbiology - 1 Oct 2009
Wolk Donna M, Blyn Lawrence B, Hall Thomas A, Sampath Rangarajan, Ranken Raymond, Ivy Cristina, Melton Rachael, Matthews Heather, White Neill, Li Feng, Harpin Vanessa, Ecker David J, Limbago Brandi, McDougal Linda K, Wysocki Vicki H, Cai Mian, Carroll Karen C
Abstract excerpt
There are few diagnostic methods that readily distinguish among community-acquired methicillin (meticillin)-resistant Staphylococcus aureus strains, now frequently transmitted within hospitals. We describe a rapid and high-throughput method for bacterial profiling of staphylococcal isolates. The method couples PCR to electrospray ionization-mass spectrometry (ESI-MS) and is performed on a platform suitable for...
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