Article
Hoechst 33342 side population identification is a conserved and unified mechanism in urological cancers.
Stem cells and development - 1 Dec 2009
Oates Jeremy E, Grey Benjamin R, Addla Sanjai K, Samuel Joanne D, Hart Claire A, Ramani Vijay A C, Brown Michael D, Clarke Noel W
Abstract excerpt
Mutation within the adult human stem cell (SC) compartment has been proposed as a factor in the initiation and promotion of carcinogenesis. Isolation of these cancer stem cells (CSCs) has proven difficult, limiting their subsequent phenotypic, functional, and genetic characterization. We have used the Hoechst 33342 dye efflux technique to isolate an epithelial side population (SP) from genitourinary (GU) cancers,...
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