Article
Identification of side population cells from bladder cancer cells by DyeCycle Violet staining.
Cancer biology & therapy - 1 Oct 2008
She Jun Jun, Zhang Peng Ge, Wang Zi Ming, Gan Wei Min, Che Xiang Ming
Abstract excerpt
Side population (SP) cells can be used to identify putative cancer stem cells (CSC), but this technique is hampered by the requirement for an ultraviolet (UV) laser source. DyeCycle Violet reagent (DCV) is a DNA-binding dye that can be used in the common violet laser diode (VLD)-equipped flow cytometer. In this paper, we analyzed SP cells from several bladder cancer cell lines using either Hoechst 33342 or DCV...
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