Article
Rubisco oligomers composed of linked small and large subunits assemble in tobacco plastids and have higher affinities for CO2 and O2.
Plant physiology - 1 Apr 2009
Whitney Spencer Michael, Kane Heather Jean, Houtz Robert L, Sharwood Robert Edward
Abstract excerpt
Manipulation of Rubisco within higher plants is complicated by the different genomic locations of the large (L; rbcL) and small (S; RbcS) subunit genes. Although rbcL can be accurately modified by plastome transformation, directed genetic manipulation of the multiple nuclear-encoded RbcS genes is more challenging. Here we demonstrate the viability of linking the S and L subunits of tobacco (Nicotiana tabacum)...
Topics
- Carbon Dioxide
- Electrophoresis, Polyacrylamide Gel
- Enzyme Stability
- Gene Expression Regulation, Plant
- Kinetics
- Methionine
- Methylation
- Oxygen
- Phenotype
- Photosynthesis
- Plant Leaves
- Plants, Genetically Modified
- Plastids