Article
Time-resolved Fourier transform infrared study on photoadduct formation and secondary structural changes within the phototropin LOV domain.
Biophysical journal - 18 Feb 2009
Pfeifer Anna, Majerus Teresa, Zikihara Kazunori, Matsuoka Daisuke, Tokutomi Satoru, Heberle Joachim, Kottke Tilman
Abstract excerpt
Phototropins are plant blue-light photoreceptors containing two light-, oxygen-, or voltage-sensitive (LOV) domains and a C-terminal kinase domain. The two LOV domains bind noncovalently flavin mononucleotide as a chromophore. We investigated the photocycle of fast-recovery mutant LOV2-I403V from Arabidopsis phototropin 2 by step-scan Fourier transform infrared spectroscopy. The reaction of the triplet excited...
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