Article
Development and evaluation of a liquid bead microarray assay for genotyping genital human papillomaviruses.
Journal of clinical microbiology - 1 Mar 2009
Feng Qinghua, Cherne Stephen, Winer Rachel L, Balasubramanian Akhila, Lee Shu-Kuang, Hawes Stephen E, Kiviat Nancy B, Koutsky Laura A
Abstract excerpt
We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective studies, the LBMA assay was compared to the RLB assay and to the Hybrid Capture II (hc2) assay....
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