Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays.

doi:10.1016/j.ab.2008.11.040

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Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays.

Analytical biochemistry - 15 Mar 2009

Cukier Holly N, Pericak-Vance Margaret A, Gilbert John R, Hedges Dale J

Abstract excerpt

The recent implication of genomic copy number variations (CNVs) in multiple human genetic disorders has led to increased interest in CNV discovery technologies. There is a growing consensus that, in addition to the method used for detection, at least one additional technology should be employed for validation. Real-time quantitative polymerase chain reaction (qPCR) analysis, incorporating a normal (2N) copy...

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Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays.

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