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Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus Genomes

25 Dec 2008

Abstract excerpt

The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates...

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PMID: 19109473
DOI: 10.1128/jcm.01028-08

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