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Microporation Is a Valuable Transfection Method for Gene Expression in Human Adipose Tissue–derived Stem Cells

9 Dec 2008

Abstract excerpt

Stem cells are a promising resource for gene therapy. Adipose tissue-derived stem cells (ADSCs) offer advantages because of their abundance and ease of isolation. However, it is difficult to transduce genes into ADSCs by common transfection methods, especially nonviral methods. We report here the use of a new electroporation method, termed "microporation," to transduce plasmids into human ADSCs (hADSCs). We...

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PMID: 19066595
DOI: 10.1038/mt.2008.267

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