Article
Serum inhibition of proliferation of serum-free mouse embryo cells.
Experimental cell research - 1 Jan 1991
Rawson C, Loo D, Helmrich A, Ernst T, Natsuno T, Merrill G, Barnes D
Abstract excerpt
Serum-free mouse embryo (SFME) cells, derived in medium supplemented with insulin, transferrin, high density lipoprotein, epidermal growth factor, and fibronectin, do not undergo crisis, maintain a predominantly diploid karyotype with no detectable chromosomal abnormalities for well over 100 population doublings in vitro, and are growth inhibited by concentrations of serum that are growth-stimulatory for most...
Topics
- Amino Acids
- Animals
- Cell Division
- Cells, Cultured
- Culture Media
- Embryo, Mammalian
- G1 Phase
- Genetic Variation
- Growth Inhibitors
- Hydrocortisone
- Interferon-gamma
- Karyotyping