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Article

Protein and electrode engineering for the covalent immobilization of P450 BMP on gold.

Analytical chemistry - 15 Nov 2008

Ferrero Valentina E V, Andolfi Laura, Di Nardo Giovanna, Sadeghi Sheila J, Fantuzzi Andrea, Cannistraro Salvatore, Gilardi Gianfranco

Abstract excerpt

Site-directed mutagenesis and functionalization of gold surfaces have been combined to obtain a stable immobilization of the heme domain of cytochrome P450 BM3 from Bacillus megaterium. Immobilization experiments were carried out using the wild type protein bearing the surface C62 and C156 and the site-directed mutants C62S, the C156S, and the double mutant C62S/C156S (no exposed cysteines). The gold surface was...

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PMID: 18947200
DOI: 10.1021/ac8011413

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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