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Article

Linkage mapping and comparative genomics using next-generation RAD sequencing of a non-model organism.

PloS one - 26 Apr 2011

Baxter Simon W, Davey John W, Johnston J Spencer, Shelton Anthony M, Heckel David G, Jiggins Chris D, Blaxter Mark L

Abstract excerpt

Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals. This approach has broad potential for genetic analysis of non-model organisms including genotype-phenotype association mapping, phylogeography, population genetics and scaffolding genome assemblies through linkage mapping. We constructed a RAD library using genomic DNA from a...

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PMID: 21541297
DOI: 10.1371/journal.pone.0019315
PMCID: PMC3082572

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