Article
Identification of a New α1,2-Fucosyltransferase Involved in O-Antigen Biosynthesis of <i>Escherichia coli</i> O86:B7 and Formation of H-Type 3 Blood Group Antigen
9 Oct 2008
Abstract excerpt
Escherichia coli O86 possesses high human blood group B activity because of its O-antigen structure, sharing the human blood group B epitope. In this study, the wbwK gene of E. coli O86:B7 was expressed and purified as the GST fusion protein. Thereafter, the wbwK gene was biochemically identified to encode an alpha1,2-fucosyltransferase through radioactivity assays, as well as mass spectrometry and NMR...
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