Article
Detection and identification of baculovirus pesticides by multitemperature single-strand conformational polymorphism.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes - 1 Sept 2008
Szewczyk Boguslaw, Barski Piotr, Sihler William, Rabalski Lukasz, Skrzecz Iwona, Hoyos-Carvajal Liliana, de Souza Marlinda Lobo
Abstract excerpt
The method of single-strand conformational polymorphism (SSCP) was modified in our laboratories for the characterization of baculoviruses, insect viruses with great potential for use as bioinsecticides in biological protection programs. A series of primers were synthesized after the comparison of the polyhedrin gene sequences of over 20 baculoviruses. Polyhedrin is a highly conserved protein which is responsible...
Topics
- Animals
- Baculoviridae
- Genetic Variation
- Insecta
- Pest Control, Biological
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Species Specificity
- Viral Structural Proteins