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Article

Development of a quantitative real-time PCR for determination of genotype frequencies for studies in baculovirus population biology.

Journal of virological methods - 1 Mar 2008

Zwart Mark P, van Oers Monique M, Cory Jenny S, van Lent Jan W M, van der Werf Wopke, Vlak Just M

Abstract excerpt

Two bacmid-derived Autographa californica Multiple-capsid Nucleopolyhedrovirus genotypes - that differ only in a short tag sequence for differential PCR recognition - were generated. By electron microscopy, these genotypes were found to have identical polyhedra morphology. Mixtures of quantified polyhedra were made and used to validate a SYBR Green I-based quantitative real-time PCR (qPCR) to determine genotype...

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PMID: 18082274
DOI: 10.1016/j.jviromet.2007.10.022

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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