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Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos

24 Jul 2009

Abstract excerpt

BACKGROUND: Recently, we demonstrated that single blastomeres of a 4-cell stage human embryo are able to develop into blastocysts with inner cell mass and trophectoderm. To further investigate potency at the 4-cell stage, we aimed to derive pluripotent human embryonic stem cells (hESC) from single blastomeres. METHODS: Four 4-cell stage embryos were split on Day 2 of preimplantation development and the 16...

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PMID: 19633307
DOI: 10.1093/humrep/dep262

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