Article
Use of a library of mutated Maackia amurensis hemagglutinin for profiling the cell lineage and differentiation.
Proteomics - 1 Aug 2008
Maenuma Keisuke, Yim Mijung, Komatsu Kunimitsu, Hoshino Mayumi, Takahashi Yoriko, Bovin Nicolai, Irimura Tatsuro
Abstract excerpt
Thirty-five variant lectins were prepared by mutations of two amino acids within the carbohydrate-recognition domain of Maackia amurensis hemagglutinin (MAH). Each lectin showed unique carbohydrate specificity according to their bindings to soluble polyacrylamide with various mono- and oligosaccharides and to glycophorin A. The relative intensity of the bindings of carcinoma, myeloid, fibroblastic, and melanoma...
Topics
- Animals
- Binding Sites
- Cell Differentiation
- Cell Line
- Cell Line, Tumor
- Cell Lineage
- Cluster Analysis
- HL-60 Cells
- Humans
- Maackia
- Mice
- Mutation
- NIH 3T3 Cells
- Plant Lectins
- Protein Binding
- U937 Cells