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Article

Experimental approaches to kinetics of gas diffusion in hydrogenase.

Proceedings of the National Academy of Sciences of the United States of America - 12 Aug 2008

Leroux Fanny, Dementin Sébastien, Burlat Bénédicte, Cournac Laurent, Volbeda Anne, Champ Stéphanie, Martin Lydie, Guigliarelli Bruno, Bertrand Patrick, Fontecilla-Camps Juan, Rousset Marc, Léger Christophe

Abstract excerpt

Hydrogenases, which catalyze H(2) to H(+) conversion as part of the bioenergetic metabolism of many microorganisms, are among the metalloenzymes for which a gas-substrate tunnel has been described by using crystallography and molecular dynamics. However, the correlation between protein structure and gas-diffusion kinetics is unexplored. Here, we introduce two quantitative methods for probing the rates of...

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Identifiers

PMID: 18685111
DOI: 10.1073/pnas.0803689105
PMCID: PMC2516266

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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